B. Depreter PharmD, E. Dumoulin PharmD, J. Billiet MD, B. Cauwelier MD, PhD, B. Maes MD, PhD, C. Matthys MD, J. Van Droogenbroeck MD, PhD, F. Nollet PhD, J. Emmerechts MD, PhD
Summary
We report a rare case of biclonal biphenotypic B-cell lymphocytosis with chronic lymphocytic leukaemia phenotype. Clonality was initially misjudged by immunophenotyping because of its polyphenotypic pattern. Polymerase chain reaction analysis revealed clonality of the immunoglobulin heavy chain and kappa light chain gene rearrangement, indicating the presence of a monoclonal B-lymphocyte population. Immunophenotyping was repeated after cell sorting and revealed two CD5+CD19+ populations with different light chain restriction. Different genetic abnormalities for both clones, as evidenced by fluorescence in situ hybridisation and hypermutation analysis, support the diagnosis of two independently originated but co-existing B-cell clones. This case illustrates the importance of using multiple techniques in the diagnostic work-up of haematological malignancies.
BJH - 2014, issue Abstract Book BSTH, november 2014
A.S. De Vriese , R. Caluwé , E. Bailleul , D. De Bacquer , D. Borrey , S. Ameye , E. Hysselinckx , B. Van Vlem , S.J. Vandecasteele , J. Emmerechts MD, PhD
BJH - volume 5, issue Abstract Book BHS, january 2014
F. Nollet PhD, K. Boon , J. Emmerechts MD, PhD, S. Vermeire , R. D’Hondt , A. Van Hoof MD, PhD, D. Selleslag MD, J. Van Droogenbroeck MD, PhD, T. Lodewyck MD, J. Billiet MD
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